Tomato (Solanum lycopersicum L.) is one of the main horticultural crops in the world. This is because of the important economic benefits derive from it. Tomato probably arrived in the eastern Mediterranean area, from Peru and Central America, at the end of the eighteen century (Pitrat, 2012). Today in Greece, there is a wide range of phenotypic and genetic variability in tomato, empirically observed across local populations. When initiating a project of molecular identification of Greek ecotypes of Solanum lycopericum by microsatellite profiling, we had in mind the origin of this genetic material. SSR-based characterization of a wide selection of tomato genotypes is genotyped with eleven SSR loci in a first effort to document the genetic diversity of tomato germplasm in Greece. The databases will be upgraded permanently along with the production and publication of new data.